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SDS-PAGE explained - Protein Separation Technique
Hey Friends, SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) is used to separate proteins in a sample according to their molecular weight. SDS binds to linearised proteins and masks the native charge. 1.4 g SDS bind per 1g protein, so that all proteins have a nearly similar mass-to-charge ratio. After an SDS-PAGE, the gel ...
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